Bio 451
Molecular Biology Techniques

Topics

Solution Preparation
Nature of Peptides and Proteins; methods of protein urification.
Chromatography
Electrophoresis: PAGE, isoelectric focusing, 2D gels, Agarose, Capillary
Electrophoresis: Analysis, Recovery of Macromolecules
Centrifugation: Types of centrifugation techniques
Centrifugation: Rotors, Tubes, Gradient materials
DNA Analysis: Electrophoresis, Restriction Enzymes, PCR
DNA Analysis: Blotting and Hybridization.
DNA sequencing
Radioisotopes and radioligands
Radioactive Labeling and Counting; Autoradiography