Bio 311

Sexually Transmitted Diseases

 

Lecture 7

 

Sexually Transmitted Bacterial Diseases

 

Information for this section was taken from the URLs indicated in each section and from the following books:

Alcamo, I.E. 2001. Fundamental of Microbiology. Jones and Bartlett Publishers, Sudbury, MA.

Wistreich, G.A. 1992. The Sexually Transmitted Diseases: A Current Approach. Wm. C. Brown Publishers, Dubuque, IA.

 

Of the STDs that are diagnosed, only four are required to be reported to state health departments and the Centers for Disease Control (CDC) for statistical purposes.  These are: Gonorrhea, Syphilis, Chlamydia and Hepatitis B.  Of these, Hepatitis B is the only infection caused by a virus, the other diseases are caused by bacteria.  Of the four, chlamydia is more prevalent in the US followed by Gonorrhea. 

 

Biology of Bacteria

1.     Bacteria are prokaryotes. Cellular organisms that do not have a nucleus.

2.    Bacteria vary in size, but are about 5 times smaller that eukaryotic cells (nucleated cells making up the human, animal and plant organisms).

3.    Bacteria are about 10 times larger than viruses.

4.    Bacteria are characterized by having the following structures:

a.              Nucleoid- Is where the genomic circular DNA of the bacteria exists.

b.             Plasmid- Is a small circular DNA that contain few genes, sometimes resistance genes.

c.              Flagella-they propel the bacteria to different places.

d.             Pili-enable attachment of bacteria to different surfaces also may allow transfer of genetic material among bacteria.

e.              Glycocalix-It is made of polysaccharides (sugars) and small proteins.  If it is thick, it is known as a capsule.  If it is thin, it is considered a slime layer.  Serves to buffer against environment and to attach to surfaces.

f.               Cell membrane*

g.             Cell wall*

* Eukaryotes also have a cell membrane that carries out similar functions, but in animal cells there is no cell wall.  Plant cells are eukaryotic and have a cell wall, but the chemical component of Plant cell walls is cellulose, the chemical component of bacteria cell wall is peptidoglycan.

 

Bacteria can be Gram-Positive or Gram-Negative

·      Gram Positive bacteria have a Thick cell wall of peptidoglycan above the cell membrane.

·      Gram Negative bacteria have a thin cell wall of peptidoglycan above the cell membrane.  In turn, an outer membrane surrounds the thin cell wall.

·      The Gram stain consists of using Crystal violet (CV) as primary staining, using iodine (I) as mordant fixation to form CV-I complex, distaining with alcohol and counterstaining with safranin to give Gram negative bacteria a pink color.

 

Bacterial Replication

Bacteria replicate by binary fission.  Bacteria can replicate in as little as 20 minutes.

 

Bacteria can have different shapes:

·      Rod (bacillus, streptobacillus)

·      Sphere (Coccus, diplococcus, staphylococcus, streptococcus)

·      Spiral (Spirillum, Spirochete, Vibrio)

 

Table I. Sexually Transmitted Bacterial Diseases

 

Disease                      Causative Agent                 Description of Agent

Syphilis                      Treponema pallidum          Spirochete

Gonorrhea                 Neisseria gonorrhoeae      Gram negative diplococcus

Chlamydia                 Chlamydia trachomatis     Chlamydia (coccus)

Ureaplasma

Urethritis                    Ureaplasma urealyticum   Mycoplasm (coccus)

Chancroid                 Haemophilus ducreyi         Gram-negative rod

Lymphogranuloma

Venereum                  Chlamydia trachomatis     Chlamydia

Granuloma

Inguinale                   Calymmatobacterium

                                    Granulomatis                       Gram-negative rod

Vaginitis                     Gardnerella vaginalis        Gram-negative rod

Mycoplasmal

Urethritis                    Mycoplasma hominis

                                    Mycoplasma genitalium    Mycoplasma

 

 

 

 

 

SYPHILIS

http://www.ashastd.org/stdfaqs/syphilis.html

 

Historical Background

Medical historians have traced syphilis from Spain throughout Europe and Asia.  One commonly held view is that syphilis was brought to Europe by Columbus and his crew after their first voyage to the West Indies.  It is true that within a few years of Columbus’s return from his first voyage to the New World, epidemics of the disease spread across Europe with devastating effects.  History suggests that the Spaniards introduced the disease to the Italians while fighting beside the troops of Alfonso II of Naples.  Then, in 1495, an army of mercenaries fighting for Charles VII of France conquered Naples.  As they returned home through France, Germany, Switzerland, Austria, and England, they carried along the disease.  By 1496, syphilis was so uncontrolled in Paris that strict laws were passed banishing anyone with the disease from the city.  In 1498, Vasco de Gama and his Portuguese crew carried syphilis to India, and from there it spread to China.  Outbreaks of syphilis in Japan soon followed the visits of European ships.

Another view holds that syphilis arrived in Spain and Portugal with slaves imported from Africa in the mid-1400s.  Another bacterial disease found in Africa, called yawa, which is quite similar to syphilis in several respects, is believed by certain historians to have flared in the form of syphilis in the late 1400s.  They further speculate that the army of Charles VIII provided a highly susceptible population that spread the disease wherever they went.

Physicians in the early sixteenth century did not have a name for the disease we now call syphilis.  However, its obvious devastating effects throughout the years inspired a number of epithets.  The Italians referred to it as the Spanish disease, while the French called it the Neapolitan disease.  As syphilis spread to many countries, it acquired the name of the French sickness, a name that lasted for about a century.

The term syphilis was introduced by the Italian physician Girolamo Fracastrorius, who wrote a poem about a shepherd boy named Syphilus.  Apparently, Syphilus left a flock of sheep belonging to the Greek god Apollo for some sexual activity.  As punishment for leaving the sheep unattended, he developed the horrible sores of the disease.

Syphilis was as international in effect as in name, and proved to respect no rank.  Henry VIII of England, Napoleon of France, and Peter the Great of Russia all contracted the disease.  Poets such as Keats, musicians such as Beethoven, and artists such as Gauguin also succumbed as did millions of common people. 

 

The Disease

Syphilis is caused by Treponema pallidum.  The bacterium spreads by human to human contact, usually during sexual intercourse. It penetrates the skin surface through the mucous membranes or via a wound, abrasion, or hair follicle.

 

·      Primary syphilis is the first stage to appear.  This stage is characterized by the chancre, a painless circular, purplish ulcer with a raised margin and hard edges described as being like cartilage.  The chancre develops at the site of entry of the spirochetes, often the genital organs.  However, any area of the skin may be affected, including the pharynx, rectum, or lips.  The chancre teems with spirochetes.  It persists for 2-6 weeks, and then it disappears spontaneously.

·      Secondary syphilis can develop 17 days to 6.5 months after infection.  Symptoms can last from 2 to 6 weeks.  The symptoms of 2nd syphilis include fever, and a constitutional flulike illness, as well a swollen lymph nodes reminiscent of infectious mononucleosis.  A rough, reddish-brown rash that appears on the palm of your hands or the soles of your feet, which normally does not itch.  Condylomata lata or syphilitic “warts”, moist, raised or elevated skin lesions, may be found in the anus or genital area, mucous patches, round, grayish white sores, can appear on the mouth, throat and cervix.  The skin rash that appears may be mistaken for measles, rubella, or chickenpox.  Loss of the eyebrows often occurs, and patchy loss of hair results in “moth-eaten” areas commonly seen on the head.  Involvement of the liver may lead to jaundice and suspicion of hepatitis.  In untreated patients, the symptoms last several weeks, and death may result.  Most patients recover, but they bear pitted scars from the lesions and remain “pockmarked.”  These individuals now enter a latent stage, during which they continue to be infectious.

·      Latent syphilis is defined as the time where there are no signs or symptoms of the disease.  It develops from 2 to more than 30 years after infection.  The only way to test for infection during the latent period is by blood test.  A relapse of secondary syphilis can occur once the disease has entered the latent stage.  This normally will happen during the first 2 years of latency.

·      About one third of untreated patients eventually develop tertiary syphilis. Symptoms of the late stage or tertiary syphilis can occur 2 to 30+ years after infection.  This stage occurs in many forms, but most commonly it involves the skin, cardiovascular system, and nervous system.  The hallmark of tertiary syphilis is the gumma, a soft, gummy granular lesion.  In the cardiovascular system, gummas weaken the major blood vessels, causing them to bulge and burst.  In the spinal cord and meninges, gummas lead to degeneration of the tissues and paralysis.  In the brain, they alter the patients personality and judgement and cause insanity so intense that for many generations, people with tertiary syphilis were confined to mental institutions.  It is conceivable that our ancestors failed to equate the chancre of primary syphilis with the horrible symptoms of tertiary syphilis because the stages were so distantly separated in time.  If treated during this period, gummas will usually disappear.  Though treatment at this phase will cure the disease and stop future damage to the body, it cannot repair or reverse the damage that occurred before treatment.

·      In pregnant women, the spirochetes penetrate the placental barrier after the fourth month of pregnancy, causing congenital syphilis in the fetus.  Syphilitic skin lesions and open sores may be apparent in the newborn, or symptoms may develop weeks after birth.  Affected children often suffer poor bone formation, meningitis, or Hutchinson’s triad, a combination of deafness, impaired vision, and notched, peg shaped teeth.  Late congenital syphilis has similar symptoms to tertiary syphilis in adult, though heart complications rarely occur in cases of congenital syphilis.

·      Diagnosis of Syphilis can be done by looking for antibodies for the bacteria or by testing fluid taken from lesions or swollen lymph nodes to look for antigens.

·      Penicillin is the drug of choice for the primary, secondary and latent stages of the disease, but antibiotics are ineffective in tertiary syphilis.

·      T. pallidum multiplies very slowly in the tissues, partly because of its 33 hour generation time.  This factor encourages successful therapy.

·      Shyphilis is currently among the most reported microbial disease in the US.  Statistics indicate that about 45,000 people are afflicted with the disease annually, of whom about 9000 are in the primary or secondary stage.  Some public health microbiologist believe that for every case reported, as many as nine cases go unreported. Up until 2000, the reported rate of syphilis in the US was at its lowest level since reporting began in 1941.  In 2001, the number of cases reported increased slightly by a little over 2%.

 

Gonorrhea

http://www.ashastd.org/stdfaqs/gonorrhea.html

Historical background

Gonorrhea has been and is a major problem causing such severe consequences as sterility, blindness, and in certain situations, death.  While this disease has been recognized as human affliction since ancient times, it remained for the well-known physician Galen, practicing in Rome during the golden age of Greek medicine, to give the disease its name.  He mistakenly believed that the discharge referred to a Rheos, was composed of semen, or Gonos.  Thus, the name gonorrhea, meaning the flow of seed, was coined.  One of the better-known common names for this disease, “clap,” was derived from the term clappoir used for Parisian houses of prostitution in the Middle Ages.

Syphilis and gonorrhea were considered to be one and the same disease for almost 300 years.  The characteristic discharge of gonorrhea was considered to be the first symptom of syphilis.  It was not until the tragic self-inflicted exposure by the English surgeon John Hunter in 1767 that it was found that there were two diseases and not one.  Hunter obtained pus from a patient with gonorrhea and injected himself.  Unfortunately, the specimen was contaminated with the T. pallidum, and Hunter fell victim to the effects of both diseases.  He subsequently developed a classic case of syphilitic heart disease and died in 1793.  It is interesting to note that even though syphilis and gonorrhea were shown to be 2 separate diseases in the 1790s, many physicians still considered them to be the same for another 50 years.

Gonorrhea is the second most frequently reported microbial disease in the US, after chlamydia.  Gonorrhea remains an epidemic.

 

The disease

·      In males, gonorrhea occurs primarily in the urethra.  Onset usually is accompanied by a tingling sensation in the penis, followed in a few days by pain when urinating.  There is also a thin, watery discharge at first, and later a more obvious whitened, thick fluid that resembles semen.  Frequent urination and an urge to urinate develop as the disease spreads further into the urethra.  The lymph nodes of the groin may also swell, and sharp pain may be felt in the testicles.  Unchecked infection of the epididymis may lead to sterility.  Symptoms tend to be more acute in males then in females.

·      Most men exhibit symptoms within 2 to 5 days after exposure, with a possible range of one to 30 days. 

 

Gonorrhea does not restrict itself to the urogenital organs. 

·      Gonococcal pharyngitis may develop in the pharynx if bacteria are transmitted by oral-genital contact; patients complain of sore throat or difficulty in swallowing. 

·      Infection of the rectum, or gonococcal proctitis, is also observed, especially when practicing anal intercourse. 

·      Transmission to the eyes may occur by fingertips or towels, and keratitis may develop.

·      Gonorrhea is particularly dangerous to infants born to infected women.  The infant may contract gonococci during passage through the birth canal and develop gonococcal opthalmia.  The most common symptoms in newborns include conjunctivitis and pneumonia, which usually develop 5-12 days after birth.

 

Diagnosis

Detection of gonorrhea can be done by bacterial DNA amplification, Gram stain test (mostly for men), culture test (very relieable test).

People infected with gonorrhea are often co-infected with chlanmydia; therefore, treatment for gonorrhea and chlamydia is prescribed.

The treatment for gonorrhea includes the following:

Cepahlosporin drugs or Quinolone class drugs.  For Chlamydia, doxycycline, azithromycin.

 

An attack of gonorrhea does not immunize one to future attacks, apparently because the immune system does not response strongly enough to the first attack.  There is no vaccine available.

 

Chlamydia

http://www.ashastd.org/stdfaqs/chlamydia.html

In the US, chlamydia is the most common bacterial STD. In 2000, 702,093 cases of chlamydia were reported to the CDC.  However, it is estimated that 3 million cases actually occurred and as many as 1 in 10 adolescent females test positive for chlamydia.

 

Until the 1950s the Chlamydia were considered to be viruses, or virus-like because of their extremely small size, and atheir need for living cells in order to multiply.  With improvements in technology and a better understanding of their properties, the chlamydia are now considered to be bacteria and not viruses.  The reasons for this decision include their cellular nature, and a susceptibility to antibiotics.

The chlamydia are distinguished from all other types of bacteria on the basis of an unusual life cycle in which they appear in two different forms, initial bodies (Ibs) which are mainly concerned with increasing the clhlamydial population in an infected cell, and elementary bodies (Ebs) which represent the infectious stage of the cycle.  An infection is started by an EB attaching to the surface of a susceptible host cell.  Once inside, the pathogen is not protected from the defenses of the host and is free to form Ibs which reproduce rapidly and eventually fill the cell.   Within 20 hrs after infection the Ibs reorganize and develop into infectious Ebs, which are released to attack and infect nearby susceptible cells.  Each infected cell may contain up to 10,000 Chlamydia.  A complete turn of the cycle from beginning to end takes 35-48 hrs.  Only Ebs are adapted to survive outside of host cells.

Two major means of transmission are recognized for C. trachomatis.  These are sexual and congenital. Newborns get infections by passing through an infected birth canal.

It is estimated that up to 77% of chlamydia infections in women are asymptomatic.  These undetected and untreated cases are associated with PID.  Symptomatic women have a thick discharge consisting of mucus and pus.  Soreness in pelvic area, lower back pain, lower abdominal pain, burning sensation on urinating slight fever, frequent need to urinate may be other symptoms.  Salpingitis, infertility, urethritis, uterine bleeding, endometritis, pregnancy complication and inflammation involving the liver are complications that may result from Chlamydia infection.

C. trachomatis is known to cause several different infections in men.  These include inflammation of the urethra of nongonococcal urethritis (NGU), a complication of chlamydial infections, an inflammation of the epididymis, or epididymitis, and an inflammation of the rectum and anus known as proctitis.  A number of STD agents and other pathogens can also cause infection of the rectum and anus.  NGU is most frequently caused by C. trachomatis.  The bacterium ureaplsma urealyticum is another cause of NGU.  Since more than half of the men with NGU are without symptoms, or experience only a mild discomfort, medical care often is not sought.  The most common symptom is the presence of a thick pus-like discharge that develops about 3 weeks after contact with an infected person.  Left untreated, NGU may lead to epididymitis and sterility. Arthritis is a common aftereffect of sexually acquired NGU.  It typically develops 1-4 weeks after the initial infection.

In Newborns infected while passing through the birth canal, ophthalmia neonatorum, pneumonia, and middle ear infections may result.  Studies indicated that 18-44% of infants born to infected mothers are likely to develop an eye infection and 11-20% are likely to develop pneumonia.

 

The disease

·      Chlamydia is a gonorrhealike disease transmitted by sexual contact.  The causative agent is Chlamydia trachomatis, a species of chlamydiae.  Chlamydia trachomatis is very small 0.25µm in diameter.  It grows only in living tissue and it has a complex reproductive cycle.  The organism appears to be a specific parasite of humans.

·      The disease has an incubation period of about 1-3 weeks, and the symptoms are remarkably similar to those of gonorrhea, although somewhat milder.

·      Approximately, 75% of women and 50% of men do not experience symptoms.  If a person does have symptoms, they usually develop within one to 3 weeks after exposure to chlamydia. 

·      The symptoms of chlamydia are similar to the symptoms of gonorrhea and the 2 infections are often confused.

·      Both men and women can experience proctitis (inflamed recturm), urethritis (inflamed urethra) and conjunctivitis (inflamed eyelid).  Most infections of the mouth and throat are asymptomatic.  If present, symptoms are soreness and redness in the throat or mouth.  The most common complications in newborns include conjuctivitis and pneumonia.

·      Most women are asymptomatic, but if symptoms are present they may be minor.  Symptoms may include: vaginal discharge, burning sensation during urination. If the infection spreads to the fallopian tubes, women may experience lower abdominal and lower back pain, pain during intercourse, bleeding between menstrual periods, nausea or fever. Females often note a slight vaginal discharge, as well as inflammation of the cervix.  Burning pain is also experienced on urination, reflecting disease in the urethra.  In complicated cases, the disease may spread to the Fallopian tubes, causing adhesions that block the passageways (salpingitis).  Some think that PID is a more likely consequence of chlamydia than of gonorrhea.  Often, there are few symptoms of disease before the salpingitis manifests itself, thus adding to the danger.

·      Men may be asymptomatic or symptoms may be minor.  When symptomatic, men may experience one or more of the following: pus or watery or milky discharge from the penis, pain or burning during urination, pain or swelling of the testicles. In males, chlamydia is characterized by painful urination and a discharge that is more watery and less copious than in gonorrhea.  The discharge is often observed after urinating for the first time in the morning.  Tingling sensations in the penis are generally evident.  Inflammation of the epididymis may result in sterility, but this complication is uncommon.

 

Diagnosis

Diagnosis consist of nucleic acid amplification test, chlamydia culture, antibody test to test for chlamydia antibodies, direct florescent antibody test-test that detect chlamydia antigens.

 

Treatment

Tetracycline is an effective treatment.  In pregnant women, erythromycin is the treatment.

 

Complications

·      Untreated chlamydia infections in women may lead to PID.  Untreated chlamydia in men may lead to prostitis (inflammation of the prostate gland) urethral scarring, infertitlity, epididymitis.

·      Chlamydial pharyngitis and proctitis are also possible.

·      Reither’s Syndrome (RS) is a disorder that causes 3 seemingly unrelated symptom-arthritis, redness of the eyes, and urinary tract problems.  Chlamydia trachomatis is one of the bacteria that can cause RS.  Most men and women with chlamydia do not develop RS.  RS usually affects men between the ages of 20 and 40.  Women can develop the disorder, though less often than men and with symptoms that are milder and less noticeable.

·      Newborn may contract C. trachomatis from an infected mother and develop a disease of the eyes known as chlamydial opththalmia.  Chlamydial pneumonia may also develop in newborns from an exposure to C. trachomatis during birth.  Thus, untreated chlamydia in infants can lead to blindness, complication of pneumonia, which can include death.

 

Lymphogranuloma Venereum

Throughout most of its history Lymphogranuloma Venereum (LGV) has been confused with other STDs, particularly syphilis, genital herpes, and chancroid.  LGV is caused by specific strains of Chlamydia trachomatis.  As with other chlamydial infections, LGV is almost exclusively spread through sexual contact.

The disease appears to be more common in tropical parts of the world such as Southeast Asia, Africa, South America, and the West Indies.  In the US, LGV predominately is found among African-American persons with a low economic status.

The most frequent sign of LGV infection and the main reason an infected person seeks medical attention is the increasing number of swollen lymph nodes in the groin.  These develop 2-6 weeks after infection, and because of anatomical differences are found more commonly in men than in women. 

·      In the primary stage, there is the appearance of a small red spot or shallow sore.  Such primary lesions also may be found on the fingers, tongue, or around the rectum and anus.  The location of lesions reflects the type of exposure and the first site of infection. 

·      The secondary stage is marked by noticeable, enlarged, hard, and painless lymph nodes on one side of the groin.  This condition generally develops within 1-3 weeks after the first signs of the infection disappear.  As this stage continues the lymph nodes on both side of the groin may become involved and give rise to a diagnostic physical feature known as the “groove sign.”  In some cases these enlarged lymph nodes may get smaller in size, break, and develop into pus-draining sores.  Fever, chills, headaches, and muscular aches are typical symptoms found with this stage of LGV infection.

·      The tertiary stage includes a variety of serious and destructive effects resulting from the spreading of the infection.  Many of these effects may be disabling as well as disfiguring, and include a narrowing of the rectum, the formation of abnormal passages or tunnels between the rectum and vulva, deep sores, and severe swelling of the genitalia resulting in the condition known as elephantiasis.

 

Diagnosis

Enzyme tests, microscopic staining techniques, culture procedures, and detection of antibodies to the organism are used in diagnosing the causative agent.

 

Treatment

Antibiotics such as tetracyclines are used as treatments.  Advanced forms of LGV may require surgery to lessen the effects of the infection or to correct any deformities.

 

Ureaplasmal Urethritis

Ureaplasmal urethritis is another type of nongonococcal urethritis.  It is caused by Ureaplasma urealyticum, a mycoplasma, so-named because of its ability to digest urea in culture media.  At about 0.15µm in size, U. urealyticum is one of the smallest known bacteria that cause human disease.

The symptoms of ureaplasmal urethritis are similar to those of gonorrhea and chlamydia.  A distinction can be made between the diseases because in ureaplasmal urethritis, the discharge is variable in quantity and the urethral pain is usually aggravated during urination.  Symptoms are often very mild.  Transmission is generally by sexual contact.

Infertility is one consequence of ureaplasmal urethritis because low sperm counts and poor movement of sperm cells have been observed in males.  Salpingitis in females has also been described.  Moreover, Ureaplasma is capable of colonizing the placenta during pregnancy, and reports have linked it to spontaneous abortions and premature births.  Twenty five per cent of NGU cases may be ureaplasmal urethritis.

 

Treatment

Penicillin cannot be used to treat ureaplasmal urethritis because U. urealyticum has no cell wall. Tetracycline is currently the drug of choice. 

 

Diagnosis

Diagnosis often depends on eliminating gonorrhea or other types of NGU as possibilities, and for this reason, cases are not often recognized.

 

 

Chancroid

http://www.ashastd.org/stdfaqs/chancroid.html

chancroid is believed to be more prevalent worldwide than gonorrhea or syphilis. 

Chancroid or soft chancre has been recognized as a disease for centuries.  It was, and often still is, confused with other STDs including syphilis.

Chancroid is a sexually transmitted infection caused by Haemophilus ducreyi.  Men have a much higher incidence of this disease than women.  Moreover, chancroid is more commonly found in uncircumcised men.

Chancroid is found worldwide, but appears to be most common in tropical and subtropical countries.  This STD is believed to be an infection of individuals with relatively low standards of cleanliness.

The incubation period for chancroid in males varies from 1-14 days following sexual contact.  In females, this period is somewhat longer for reasons that are not clear.

Typical lesions or chancroidal sores first appear at the sites of sexual contact.  These lesions which begin as small red pimples, develop into sores or ulcers within 24 h.  Classic chancroidal ulcers, which occur in the majority of men, generally are painful, irregular in shape, soft, and sharply outlined.  A foul odor occurs with some infections.

The most common locations for such lesions in males include the internal surface of the foreskin, glans, penis, and shaft of the penis.  The scrotum, anus, and thighs also may be involved.  In women, lesions develop on the labia, clitoris, vestibule, and anus.  While extragenital ulcers are rare, they can occur on fingers, breasts, lips, the tongue, etc.

Within one week after the ulcer forms, the lymph nodes in the groin generally become swollen in 40% of men and 25% of women.  If left untreated, these lesions develop into deep, pus producing sores.

 

Diagnosis

Diagnosis is made by isolating the bacteria Hemophilus Ducreyi in a culture from a genital ulcer. The chancre is often confused with syphilis, herpes or lymphogranuloma venereum

 

Treatment

Antibiotics are the treatment (check weblink)

 

Granuloma Inguinale

 

Granuloma inguinale results from infection with Calymmatobacterium granulamatis.  Sexual contact is considered to be the means of disease transmission.

The incubation period ranges from a few days to 4 or 5 months.  In most cases of infection, the main lesion, a firm red pimple or papule, develops on the genitalia.  Additional papules soon occur, eventually break open, and become sores of varying sizes and appearances.  While many such ulcers are painless, others are tender, bleed on touch, and cause disfiguring and even destruction of genitalia.  In men, for example, if left untreated, the infection may destroy the entire penis.  Long-lasting infections in women may result in severe swelling of and the formation of growths of the external genitalia.

 

Diagnosis

The finding of the characteristic genital lesions, and demonstrating the causative agent in specimens taken from the infected person are the diagnostic measures.  In the laboratory the infection is confirmed by showing the presence of Donovan bodies.  These are large, nucleated cells containing the causative bacteria.

 

Treatment

Antibiotics such as ampicillin or the tetracyclines are used.

 

Bacterial Vaginosis

http://www.ashastd.org/stdfaqs/bv.html

http://www.ashastd.org/stdfaqs/vaginitis.html

Vaginal infection can be caused when the normal flora is out of balance.  Such conditions, which include pregnancy, menstruation, and the use of oral contraceptives, change the acid level and sugar concentration in the vagina.  Several microorganisms are able to take advantage of the situation and establish an infection.  One of these, the bacterium Gardnerella vaginalis interacts with other bacterium to cause bacterial vaginosis (BV).  Thus, unlike other STDs, BV is not cause by a single infectious agent.

G. vaginalis currently is involved with about 50% of vaginosis cases.  Men occasionally get an infection of the penis after sexual contact with a woman who has vaginosis.  The disease agent can be spread to newborn passing thought an infected birth canal.

The major complaint expressed by infected women is the presence of a foul-smelling, or fishy, vaginal odor.  This odor may increase after sexual intercourse or menstruation.   Infected men also may have discharge.

 

Diagnosis

The diagnosis of bacterial vaginoiss caused by G. vaginalis is made on the physical finding of a foul smelling, or fishy discharge and the microscopic examination of a vaginal discharge in the laboratory.  When potassium hydroxzide is added to vaginal secretions, a distinct and intense fishy odor is produced.  This is a conclusive test for diagnosis.  Microscopic examination of specimens showing the surfaces of vaginal cells overrun by G. vaginalis also is used to confirm the diagnosis. 

 

Treatment

Prescription medication, usually antibiotics, depending on the organism causing the infection is used to treat and/or cure BV.