BIO 421 – Advanced Genetics

Fall 2009

Course Syllabus

Instructor and Course Information

Instructor:   Dr. Craig W. LaMunyon

Lectures:     Mon/Weds   2:00 – 3:50 pm, Bldg. 1, rm 109

Office:           Bldg. 4, rm. 426           

Phone:         (909) 869-2273

Email:           cwlamunyon@csupomona.edu

Office Hours:          Mon/Weds 12:00 - 1:30 pm or by appointment

Web site:     http://www.csupomona.edu/~cwlamunyon/BIO421/BIO421syl.htm

Text:              Genetics: Analysis and Principles by Brooker, 3rd Edition, McGraw Hill 2009

Course Description: 

Modern Genetics resides at the intersection of a number of disciplines, including molecular and cell biology, genomics and proteomics, developmental biology, evolutionary biology, agriculture, and medicine.  Whereas classical geneticists were consumed with the construction of linkage maps to understand the transmission of traits across generations, todayÕs geneticists are identifying the DNA sequence of those mutated genes, studying the functions of the encoded proteins, determining sets of genes that interact on a molecular basis, and dozens of other fascinating aspects of modern genetics.  Indeed, genetics today is really a tool - or set of tools - used in many areas of biology.  The problem with genetics as it relates to a course is that it is just too huge to fully cover in one term, especially a quarter!  I am truly sorry that we will only touch on several topics and leave others untouched.  However, with the preparation that you have received in BIO303 and BIO310, we should be able to make some real headway.  Therefore, I will assume that you already know the following topics: Mitosis and Meiosis, Mendelian and non-Mendelian inheritance, recombination, DNA structure and replication, gene expression and its control, and mutation.

Use of the textbook in this course. While I cannot possibly cover everything in the textbook, you should nonetheless read and attempt to understand all the material in the readings.  They will provide you with the background necessary to understand the lectures.  Please read the chapters before coming to class; it will make you an informed student, and you will be more likely to excel.  The text is not designed for an advanced course.  In fact, I am using the text most instructors use in BIO303 -  to save you the cost of purchasing another text (if you didnÕt already sell it back to the bookstore!).  While genetics may not be the most readable material, this textbook is one of the most readable in its field.  There may be readings added to what I assign from the textbook. 

In this course, I anticipate that you will learn the following: 

1.       Gene mapping using classical and modern molecular techniques

2.       Quantitative trait analysis and mapping

3.       Mutation analysis

4.       Reverse genetics

5.       Cytoplasmic inheritance

6.       Bacterial genetics and gene mapping

7.       Genomics and Proteomics

8.       Population genetics

9.       Evolutionary genetics

To further your learning experience in this course, I encourage you to interrupt me with questions - either if you cannot follow my arguments or if you want to challenge them (science requires alternative opinions/hypotheses!).

Pre-requisites

BIO303 (Genetics)

Course Website

The lecture schedule will be posted on the course website.  It will be updated for each lecture, and you will find outlines there for each lecture.  Also, I will post images from my PowerPoint presentations on the website

Exams and Grading

There will be three exams in this course: 2 midterms and a final that is not comprehensive.  Each exam covers approximately 5 lectures.  The exams are of equal value: 100 points each.  The exams will have both multiple choice and written questions.  Please bring a ScanTron form 882.  In addition, there will be 20 pts of extra credit given out over the quarter.  This may be in the form of quizzes and/or problem sets.  Your grade will be based on 300 points, even though 320 points are possible over the quarter. 

There will be no make-up exams.  Arrangements can be made in extreme cases, but you must alert me before the exam. 

 

CSU Employee Furloughs – Impact on Classes

This year across this campus and around the CSU system some class days will be cancelled because of furloughs. A furlough is mandatory un-paid time off; faculty and staff on each CSU campus are being ÒfurloughedÓ two days per month.

These cancelled class days are marked on your syllabus below. It is important to recognize that these days off are not holidays. Instead, they are concrete examples of how massive state budget cuts have consequences for you as students and for me as a faculty member.

The CSU has suffered chronic underfunding for at least 10 years. This year the budget cuts are the worst in the history of our university system — $584 million or 20% of our budget.  The CSU administration is attempting to deal with these cuts with huge increases in your student fees (32%), eliminations of your classes, and lay-offs of faculty and other university employees.  In addition to paying higher fees, you will be affected by reduced services and classes. The library will have shorter hours. Many campus support services will be decreased or eliminated. It will be more difficult to get signatures to meet deadlines. Classes you need may have been cut from the class schedule or are full.

 

 

Tentative Lecture Schedule

Because this is the first time this course has been offered in a 4 unit format, the lecture schedule is highly tentative. Please refer to the schedule online for the future.  It will give you the up-to-date topics and readings as they will surely change.

 

Lecture

Date

Topic

Chapter

1

Sept 28

Course Introduction; review of Mendelian and non-Mendelian genetics

Powerpoint images

1, 4

2

Sept 30

Finish review of Mendelian and non-Mendelian Genetics

Powerpoint images

1, 4

3

Oct 5

Discovering genes by Mutational analysis (Please use the outline from the last lecture)

Powerpoint images

5

4

Oct 7

Finish Gene Discovery, begin Genetic mapping

Powerpoint images

5

 

Oct 12

Furlough Break: 

Problem Set

For those of you who would like to look a first-hand description of mutagenesis, read the paper by Brenner from 1974.  It is the first paper to address C. elegans genetics.  There is a section on isolation of mutants (p 74-75), but you may find that you want to read on!

You can find a short discussion of suppression analysis on pages 92-93 in your textbook.

Finally, I have scanned the overheads from lectures 3 and 4:

Overheads

Remember that the overheads wonÕt make a lot of sense without your notes (and memories!) from lecture.

 

Please note:  There is now a copy of the text on reserve in the library

5

Oct 14

Finish Genetic Mapping

Powerpoint images

Overheads

Problem set answers due!

Answers for the Problem Set

Practice Questions for the exam

Update on 10/18:  Answers for the practice questions

 

 

Oct 19

Exam 1 - Bring a Scantron form 882

Answers for the written section

 

6

Oct 21

Finish 3-factor mapping;  molecular markers in mapping: SNPs

Powerpoint images

Overheads

PCR Movie

Restriction Enzyme Movie

 

7

Oct 26

Today we finished SNP use in mapping

The SNP gel is the last slide from lecture 6

Overheads

OK, so here is another Problem Set  worth 2 extra credit points.  It deals with SNP mapping.  It is due Nov. 2.

 

8

Oct 28

Analysis of genes and begin Reverse Genetics

Powerpoint images

Overheads

 

9

Nov 2

DNA sequencing and the Human Genome

Powerpoint images

Overheads

Problem Set 2 due

Answers to Problem Set 2

503-507

10

Nov 4

Epigenetics and Cytoplasmic Inheritance

Powerpoint images

Overheads

7

 

Nov 9

Furlough Break: TBA

 

 

Nov 11

Holiday: Veterans Day

 

 

Nov 16

Exam 2 - Bring a Scantron form 882

Practice Questions for the exam

 

11

Nov 18

Cytoplasmic Inheritance and Bacterial Genetics

Ch 7, p 174-182

Ch 6, section 6.1

12

Nov 23

Finish Bacterial Genetics, begin Quantitative Genetics

25

13

Nov 25

Finish quantitative genetics

25

14

Dec 30

Population genetics

24

15

Dec 2

Evolutionary Genetics

26

 

Dec 7

Final Exam 1:40 – 3:40 pm – Bring a Scantron form 882