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Shelton Murinda

Shelton Murinda Title: Associate Professor

Department: Animal & Veterinary Sciences

Room: 2-108

Phone: (909) 869-2089

Education:

BS - The University of Zimbabwe, Zimbabwe
MS - The National University of Ireland, Republic of Ireland
PhD - The Pennsylvania State University, Pennsylvania
Post Doctoral - The University of Tennessee Food Safety Center of Excellence, Tennessee

Biography:

Food Scientist/Microbiologist with training in Molecular-Based Methods. Education, research, extension, and outreach experiences from 3 continents. Joined Cal Poly Animal & Veterinary Sciences Department in Fall 2005.
Previous research involved evaluation of lactic acid-producing Bacillus and Sporolactobacillus for activity against Listeria monocytogenes a key pathogen in ready-to-eat meat products. Evaluated colicins for inhibitory activity against diarrheagenc E. coli strains, including, E. coli serotype O157:H7and finding natural methods for their control in particular using colicins. Conducted cytotoxicity studies of colicins on cultured mammalian cells with relevance to potential use of colicins in food/feed.
Extensive postdoctoral experiences involved a focus on reduction of mastitis pathogens ( Staphyloccoccus, aureus, Streptococcus agalactiae, Streptococcus uberis ) and foodborne pathogens (Shiga toxin-producing E. coli, Listeria monocytogenes, Campylobacter jejuni and Salmonella species) from dairy farms. Conventional, rapid methods and molecular-based methods were employed. Pathogen detection and quantification involving use of PCR and Real-Time PCR.
Developing a Benign Lab (Non-Pathogen) for detection and enumeration of food spoilage microorganisms (with Dr. Maria Botero-Omary). Research projects will focus on shelf-life studies (e.g., Hispanic food products). Project will emphasize detection and quantification of spoilage microorganisms. Specifically, fungi (yeasts and molds), total viable bacteria, and coliform bacteria that degrade or adversely affect the organoleptic properties (off-colors and off-odors, sliminess, texture, etc.) of processed foods, and hence curtail shelf-life of food will be targeted. The long term goal of this collaboration is to develop effective control methods for spoilage microorganisms using natural or non-antibiotic methods.
Developing a new Foodborne Pathogen Research Laboratory at the Equine Research Center Laboratory. This lab's research will focus on pathogens of interest to the Meat and Dairy industries. Additionally, to cater for the Equine industry, research will be initiated on discovery of probiotics with special emphasis on reducing death of foals caused by toxin-producing Clostridium bacterial species which are indicated to be prevalent in this sector. Probiotics are beneficial live bacteria that are fed to animals to competitively exclude pathogens from the gastrointestinal tracts of these animals.

Research Areas/Expertise:

Diarrheagenic foodborne pathogens and control
Mastitis pathogens and control
Use of probiotics, bacteriocins/colicins for pathogen control
Virulence characteristics and genetic determinants
Rapid pathogen detection and enumeration using nucleotide-based methods
Antimicrobial resistance and genetic determinants

Refereed Professional Publications (Last 4 years):

2006

Murinda, S.E., L.T. Nguyen, S. J. Ivey, P. Meleo and S.P. Oliver. 2006. Potential of antimicrobial agents to inhibit fungi to aid isolation of Campylobacter. (Accepted).

2006

Srinivasan, V., B. E. Gillespie, L. T. Nguyen, S. I. Headrick, S. E. Murinda, and S. P. Oliver. Characterization of antimicrobial resistance patterns and Class 1 integrons in Escherichia coli O26 isolated from human and animals. Int. J. Antimicrob. Agents (S ubmitted ).

2006

Srinivasan, V., L. T. Nguyen, S. I. Headrick, S. E. Murinda , and S. P. Oliver. Antimicrobial resistance patterns of Escherichia coli O157:H7 and O157 from dairy and humans. Microb. Drug Resist. (S ubmitted ).

2006

Srinivasan, V., L. T. Nguyen, S. I. Headrick, S. E. Murinda and S. P. Oliver. Antimicrobial resistance patterns of Escherichia coli O157, O157:H7 and non-O157 STEC from different origins. Submitted.

2006

Murinda S. E . and S. P. Oliver. Physiologic and molecular markers for detection of Shiga toxin-producing Escherichia coli serotype O26 strains: A review. Foodborne Pathog. Dis . ( Accepted ).

2005

Murinda, S. E. , K. Rashid, E. Kunze and R. F. Roberts. Novel real time method for in vitro cytotoxicity assessment of bacteriocin mode of action against mammalian cells with interactive laser image cytometry and DiOC5(3), a membrane potential. Submitted

2005

Murinda, S. E. , P. D. Ebner, L. T. Nguyen, A. G. Mathew, and S. P. Oliver. Antimicrobial resistance and Class 1 integrons in pathogenic Escherichia coli . Foodborne Pathog. Dis .2:348-352.

2005

Srinivasan, V., L. T. Nguyen, S. J. Headrick , S. E. Murinda , and S. P. Oliver. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog. Dis .2:201

2005

Nam , H. M., V. Srinivasan, B. E. Gillespie, S. E. Murinda and S. P. Oliver. Application of SYBR Green real-time PCR assay for specific detection of Salmonella spp. in dairy farm environmental samples. Int. J. Food Microbiol . 102:161-171.

2005

Srinivasan, V., H. M. Nam, L. T. Nguyen, B. Tamilselvam, S. E. Murinda , and S. P. Oliver. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog. Dis .2:201-211.

2005

Nam , H. M., V. Srinivasan, B. E. Gillespie, S. E. Murinda and S. P. Oliver. Specific detection of C ampylobacter jejuni in dairy farm environmental samples using SYBR green real time polymerase chain reaction. Foodborne Pathog. Dis . 2:160-168.

2004

Nguyen, L. T., B. E. Gillespie, H. M. Nam, S. E. Murinda , and S. P. Oliver. Detection of Escherichia coli O157:H7 and Listeria monocytogenes in beef products by real-time polymerase chain reaction. Foodborne Pathog Dis . 1:231-240.

2004

Murinda, S. E., L. T. Nguyen, T. L. Landers, F. A. Draughon, A. G. Mathew, J. S. Hogan, K. L. Smith, D. D. Hancock and S. P. Oliver. Comparison of Escherichia coli isolates from humans, food, farm and companion animals, for presence of Shiga-toxin producing E. coli virulence markers. Foodborne Pathog Dis. 1:178-184.

2004

Murinda, S. E. , L. T. Nguyen, R. A. Almeida and S. P. Oliver. Problems in isolation of Campylobacter jejuni from frozen-stored raw milk and bovine fecal samples: Genetic confirmation of isolates by multiplex PCR. Foodborne Pathog. Dis . 1:166-171.

2004

Murinda , S. E., S. D. Batson, L. T. Nguyen, B. E. Gillespie, and S. P. Oliver. Phenotypic and genetic markers for serotype-specific detection of Shiga toxin-producing E. coli O26 strains from North America . Foodborne Pathog. Dis. 1:125-135.

2004

Murinda, S. E., L. T. Nguyen, H-M. Nam , R. A. Almeida, S. J. Headrick, and S. P. Oliver. Detection of sorbitol-positive and sorbitol-negative Shiga toxin-producing Escherichia coli , Listeria monocytogenes , Campylobacter jejuni , and Salmonella spp. in dairy farm environmental samples. Foodborne Pathog. Dis. 1:97-104.

2004

Nam , H-M., Murinda, S. E., L. T. Nguyen, and S. P. Oliver. Evaluation of universal preenrichment broth (UPB) for simultaneous isolation of Salmonella spp. , Escherichia coli O157:H7 and Listeria monocytogenes from dairy farm environmental samples. Foodborne Pathog. Dis. 1:37-44.

2003

Murinda, S. E ., K. A. Rashid and R. F. Roberts. In vitro cytotoxicity of nisin, pediocin and selected colicins on Simian virus 40-transfected human colon and Vero monkey kidney cells using trypan blue staining viability assays. J. Food Prot . 66:847-853.

2002

Murinda, S. E ., L. T. Nguyen, S. J. Ivey, B. E. Gillespie, R. A. Almeida and S. P. Oliver. 2002. Molecular characterization of Salmonella spp. isolated from bulk tank milk and cull dairy cow fecal samples. J. Food Prot. 65:1100-1105.

Book Chapter :

2005

Oliver, S.P., S.E Murinda, L.T. Nguyen, H. M, Nam, R.A. Almeida, and S.J. Headrick. 2005. On-farm sources of foodborne pathogens: isolation from the diary farm environment. In: Mastitis in Dairy Production, H. Hogeveen (ed.). Wageningen Academic Publishers, The Netherlands. p665-670.

Non-Refereed Technical Publications (Last 4 years):

2005

Oliver, S.P., S.E Murinda, L.T. Nguyen, H. M, Nam, R.A. Almeida, and S.J. Headrick. 2005. On-farm sources of foodborne pathogens: isolation from the dairy farm environment. In: Mastitis in Dairy Production, H. Hogeveen (ed). Wageningen Academic Publishers, The Netherlands. p665-670.

2004

Srinivasan, V., H. M. Nam, L. T. Nguyen, B. Tamilselvam, S. E. Murinda, and S. P. Oliver. Prevalence of antibiotic resistance genes and integrons in foodborne pathogens isolated from dairy farms. In: Proc. Natl. Mastitis Counc. pp. 369-370.

2004

Murinda, S. E., L. T. Nguyen, H-M. Nam , R. A. Almeida and S. P. Oliver. Detection of Shiga toxin producing E. coli , Listeria monocytogenes , Campylobacter jejuni and Salmonella spp. from dairy farm environmental samples. In: Proc. Ntnl. Mastitis Council. pp. 351-352 .

2004

Nam , H-M., S. E Murinda , L. T. Nguyen, and S. P. Oliver. Evaluation of universal preenrichment broth (UPB) for simultaneous isolation of Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes and Salmonella spp. from dairy farm environmental samples. In: Proc. Ntnl. Mastitis Council. pp.347-348.

2004

Nam , H-M., B. E. Gillespie, S. E Murinda , and S. P. Oliver. Application of SYBR GREEN real-time polymerase chain reaction for simultaneous detection of Campylobacter jejuni , Escherichia coli O157:H7, and Salmonella spp. In: Proc. Ntnl. Mastitis Council. pp. 349-350 .

2003

Murinda, S. E., L. T. Nguyen, T. L. Landers, F. A. Draughon, A. G. Mathew, J. S. Hogan, K. L. Smith, D. D. Hancock and S. P. Oliver. Multiplex PCR for detection and confirmation of Shiga toxin-producing Escherichia coli. In: Department of Animal Science Annual Report, The University of Tennessee , Knoxville . http://www.agriculture.utk.edu/ansci/annual_reports_all.htm .

2003

Oliver, S. P., S. E. Murinda and R. A. Almeida. Mastitis control, food safety and quality milk production. In: Department of Animal Science Annual Report, The University of Tennessee , Knoxville . http://www.agriculture.utk.edu/ansci/annual_reports_all.htm .

2003

Batson, S. D., S. E. Murinda , L.T. Nguyen and S.P. Oliver. Phenotypic and genetic characterization of Escherichia coli O26 isolates from North America using carbohydrate fermentation and genes encoding virulence factors. In: Department of Animal Science Annual Report, The University of Tennessee , Knoxville . http://www.agriculture.utk.edu/ansci/annual _reports_all.htm .

2003

Murinda , S. E., L. T. Nguyen and S. P. Oliver. Genetic confirmation of Campylobacter jejuni isolated from dairy farms using multiplex PCR targeting thermophilic and hippuricase gene sequences. In: Department of Animal Science Annual Report, The University of Tennessee , Knoxville . http://www.agriculture.utk.edu/ansci/annual_reports_all.htm .

2003

Murinda , S. E., L. T. Nguyen, H-M. Nam , R. A. Almeida and S. P. Oliver. Detection of Shiga toxin-producing E. coli , Listeria monocytogenes , Campylobacter jejuni and Salmonella from dairy farms. In: Department of Animal Science Annual Report, The University of Tennessee , Knoxville . http://www.agriculture.utk.edu/ansci/annual_reports_all.htm.

2003

Batson , S.D. , S. E. Murinda , L. T. Nguyen and S. P. Oliver. Detection of Shiga toxin-producing E. coli O26 using phenotypic and genetic markers. In: Proc. Ntnl. Mastitis Council. pp. 344-345.

2003

Murinda , S.E., L. T. Nguyen, T. L. Landers, F. A. Draughon, A. G. Mathew, J. S. Hogan, K. L. Smith, D. D. Hancock and S. P. Oliver. Multiplex PCR for detection of pathogenic E. coli . In: Proc. Ntnl. Mastitis Council . pp. 348-349.

2002

Murinda, S. E. , L. T. Nguyen, T. L. Landers, F. A. Draughon, A. G. Mathew, J. S. Hogan, K. L. Smith, D. D. Hancock and S. P. Oliver. Multiplex PCR for detection and confirmation of Shiga toxin-producing Escherichia coli . In: Proceedings of the Conference of Research Workers in Animal Diseases . Abstract #53P.

2002

Batson, S. D., S. E. Murinda and S. P. Oliver. Phenotypic and genetic characterization of Escherichia coli O26 isolates from North America using carbohydrate fermentation and genes encoding virulence factors. In: Proceedings of the Conference of Research Workers in Animal Diseases . Abstract #79.

2002

Ebner, P. D., S. E. Murinda , B. G. Gillespie, S. P. Oliver and A. G. Mathew. Virulence attributes of E. coli O157:H7 isolated from dairy farms in East Tennessee . In: Proc. Intnl. Assoc. Food Prot. p88.

2002

Murinda, S. E., L. T. Nguyen, S. J. Ivey, R. A. Almeida, M. J. Montgomery, F. A. Draughon and S. P. Oliver. Isolation of mastitis and foodborne pathogens in bulk tank milk and fecal samples from cull dairy cows. In: Proc. Ntnl. Mastitis Council , pp.178-179.

2002

Murinda, S. E. , L. T. Nguyen, S. J. Ivey, R. A. Almeida, M. J. Montgomery, F. A. Draughon and S. P. Oliver. Prevalence of Salmonella in bulk tank milk and fecal samples from cull dairy cows. In: Proc. Ntnl. Mastitis Council , pp.174-175.

2002

Murinda, S. E. , L. T. Nguyen, S. J. Ivey, R. A. Almeida, M. J. Montgomery, F. A. Draughon and S. P. Oliver. Prevalence of Escherichia coli O157:H7 in bulk tank milk and fecal samples from cull dairy cows. In: Proc. Ntnl. Mastitis Council , pp.176-177.