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Remote 3-D Light Microscopic Imaging and Stage Control,  part  2

Remote Control of Motorized Stage and the 3-D Imaging of Light Microscopic Objects (Fig. 5A,B):  With the purchase of the 3-D Edge Rotating Illuminator and mounting onto the Nikon Optiphot-2 system equipped with the PRIOR motorized stage and videocamera system, it became possible to not only remotely control, view, and capture microscopic images, but also to view the microscopic images in 3-D!  The new system will allow students in remote classrooms to view microscopic images in 3-D.

Fig. 4A      Edge 3-D Microscope                B Stereoimage of a pyramidal neuron

                 

Fig. 5A Edge 3-D Rotating Illuminator                        B Illuminator Control Panel

Solid State analog cameras and video capture were evaluated for suitability to provide image capture for light microscopy.  We also evaluated 3-D video capture capabilities to allow for the acquisition and transmission of high-quality stereoscopic images in near real time (at 15 to 30 frames per second).  The production of videoimages of 3-D microscopic objects will enable us to archive important biological phenomena or experiments where 3-D imaging is useful.  Higher speed network capabilities will soon allow us to view remote 3-D microscopic video images in near real-time.

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Questions? Comments? Contact: Glenn H. Kageyama